Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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author:"Feng Y" AND citation_journal_name:"BIOCHEM BIOPHYS RES COMMUN"

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EMD-30914

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A refined cryo-EM structure of an Escherichia coli RNAP-promoter open complex (RPo) with SspA

Release date: Nov. 17, 2021
Resolution: 3.68 Å
EM Method: Single-particle
Fitted PDBs: 7dy6
Q-score: 0.474

Wang F, Feng Y, Shang Z, Lin W

Biochem Biophys Res Commun (2021) 583 pp. 86-92 [ Pubmed: 34735884 DOI: doi:10.1016/j.bbrc.2021.10.048 ]

Sample:

Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
Dna (63-mer) (19 kDa, DNA from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
An escherichia coli rnap-promoter open complex (rpo) with sspa (Cell from Escherichia coli (strain K12))
Stringent starvation protein a (24 kDa, Protein from Escherichia coli (strain K12))
Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
Dna (63-mer) (19 kDa, DNA from Escherichia coli)

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